Figure 6

Critical role of N-SMase in CAPE-induced apoptosis. (A) Inhibition of CAPE-induced caspase-3 and PARP expression by a sphingomyelinase inhibitor. C6 glioma cells were pretreated with a sphingomyelinase inhibitor (GW4869, 20 μM) for 1 h and then treated with CAPE (50 μM) for the indicated times. Cell extracts were assessed by western blot analysis with anti-procaspase-3, anti-PARP, and anti-actin antibodies. (B) C6 glioma cells were pretreated with a sphingomyelinase inhibitor (GW4869, 20 μM) for 1 hand and then treated with CAPE (50 μM) for 24 h. Apoptosis was detected with DAPI staining.