Figure 1

Expression of TFAP2c , HIF-1α mRNA and protein after the transfection of CITED2 wild-type and mutant recombinant plasmids. A: Q-PCR analysis showed TFAP2c mRNA expression levels were reduced significantly in mutant group comparing to the wild-type group in H9C2 (*P < 0.05). By contrast, HIF-1α mRNA expression levels were higher in the mutant group than that in wild- type group (*P < 0.05). B: Q-PCR analysis showed TFAP2c mRNA expression levels were reduced significantly in mutant group comparing to the wild-type group in HepG2 (*P < 0.05). By contrast, HIF-1α mRNA expression levels were higher in the mutant group than that in wild- type group (*P < 0.05). C: Western-blotting data demonstrated that TFAP2c protein concentrations were significantly lower in the mutant group comparing to the wild-type control group in H9C2 (*P < 0.05). But the concentrations of HIF-1α protein was higher in the mutant group than that of the wild-type control group (*P < 0.05). D: Western-blotting data demonstrated that TFAP2c protein concentrations were significantly lower in the mutant group comparing to the wild-type control group in HepG2 (*P < 0.05). But the concentrations of HIF-1α protein was higher in the mutant group than that of the wild-type control group (*P < 0.05).