Figure 2
From: CITED2 Mutation and methylation in children with congenital heart disease
The clone sequencing and Electrophoresis by BSP and MSP. A. The clone sequencing of reverse complementary chain in CHD and control group by BSP. a: It shows the methylated sequence in CHD group, arrow represent methylated sites; b: The corresponding non-methylated sequences in control group, arrow represent unmethylated sites. B. It shows the result of Electrophoresis by MSP. it demonstrates that Methylated strip exsits in the samples with CHD,but in the control group it shows the Unmethylated strip. It demonstrates that CITED2 methylation exsits in CHD group. 1: DNA Marker 100-600 bp, 2: Blank control (ddH2O), 3: Methylated positive control, 11: Negative control, 4-8: Methylated samples in congenital heart disease, 9-10: Unmethylated samples in the control group, M: methylated strip, U: unmethylated strip.