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Figure 1 | Journal of Biomedical Science

Figure 1

From: Expression of Nek1 during kidney development and cyst formation in multiple nephron segments in the Nek1-deficient kat2J mouse model of polycystic kidney disease

Figure 1

Expression of Nek1 during kidney development. (A, B) Nek1 is expressed ubiquitously but its expression diminishes as the mouse kidney matures. Western blots of tissue lysates. p84, a nuclear matrix protein, serves as a loading control. (C) Immunohistochemical staining for Nek1 in mouse kidneys at different ages, counterstained with methyl green to identify nuclei. Bar, 100 μm. (D) Nek1 is expressed in the cytoplasm of glomerular epithelial cells. Formalin-fixed, paraffin-embedded section was dual stained with rabbit anti-Nek1 (developed with brown- (upper corner), or purple- (lower panel) conjugated anti-rabbit IgG secondary antibody) and mouse monoclonal anti-WT-1 (developed with brown-conjugated anti-mouse IgG secondary antibody). Cytoplasmic staining for Nek1 is evident in the same cells with nuclear staining for WT-1.

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Journal of Biomedical Science

ISSN: 1423-0127