Figure 3
Thin cortical zone, increased cell death, and decreased proliferation in newborn kat2J/Nek1 −/− kidneys. (A, B) Hematoxylin and eosin (H&E)-stained wild type (A) and kat2J/Nek1−/− (B) kidney sections. Arrowheads in the upper right corner of photomicrographs of hematoxylin and eosin (H&E)-stained sections indicate the renal capsule. Note thinner, more disorganized, subcapsular, cortical zone in the representative kat2J/Nek1 −/− kidney. (C, D, G, H) Excessive apoptosis in Kat2J/Nek1−/− kidneys. Kidney sections from wild type (C&G) and kat2J/Nek1−/− mice (D&H) were analyzed by TUNEL assay. Note more TUNEL-positive, apoptotic cells in the kat2J/Nek1−/− kidney). (E, F, I, J) The proliferation status of cells in wild type (E&I) and kat2J/Nek1−/− (F&J) kidneys was analyzed by PCNA expression. Note relatively fewer PNCA-positive, proliferating cells in the kat2J/Nek1 −/− kidney. Bar, 100 μm in panels A-F; 230 μm in panels G-J.