Figure 4

Delay in kidney development and maturation in kat2J/Nek1−/− mice. Aberrant and delayed maturation of metanephric mesenchyme-derived tubules in the cortex of kat2J/Nek1 −/− kidneys. Sections of kidneys from newborn mice were immunostained with lectins marking proximal tubules (LTL) and collecting tubules (DBF), or with primary antibodies recognizing loops of Henle (THP) or collecting ducts (AQP2). They were then developed directly with immumoperoxidase or indirectly after incubation with secondary antibodies (THP and AQP2), and counterstained with methyl green. Bar, 100 μm. Sections from postnatal day 7 and 14 (P7 and P14) kat2J/Nek1−/− mice were immunostained with the same lectins or primary antibodies. They were then developed directly with immumoperoxidase or indirectly after incubation with secondary antibodies, as above, and counterstained with methyl green. Bar, 100 μm.