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Figure 5 | Journal of Biomedical Science

Figure 5

From: Expression of Nek1 during kidney development and cyst formation in multiple nephron segments in the Nek1-deficient kat2J mouse model of polycystic kidney disease

Figure 5

Delayed glomerular maturation in newborn kat2J/Nek1 −/− kidneys. Epoxy-embedded, toluidine blue stained sections of subcapsular (surface) and deeper (juxtamedullary) glomeruli from representative, littermate, wild type (A, B) and kat2J/Nek1 −/− (C, D) kidneys. Compare the more mature, wild type, juxtamedullary glomerulus in B, to the kat2J/Nek1−/− juxtamedullary glomerulus still in S-shaped form in D. Bar, 25 μm. Immunocytochemical staining of formalin-fixed, paraffin-embedded sections from the same kidneys for WT-1, a nuclear marker for metanephric mesenchyme and podocytes (E-H) confirmed that in kat2J/Nek1−/− kidneys, nascent juxtamedullary glomeruli were still in S-shaped forms (G, H). At the same time, nascent juxtamedullary glomeruli in kidneys of sex-matched, wild type, littermate mice have progressed to more mature architecture, with WT-1 staining in a ringed podocyte pattern (E, F). Bar, 20 μm.

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