Figure 1
From: ADI1, a methionine salvage pathway enzyme, is required for Drosophila fecundity
Isolation of D adi1 alleles. (A) Schematic diagram of the methionine salvage and recycling pathways, which is generated based on metabolism pathways in the Kyoto Encyclopedia Genes and Genomes (KEGG) database. The Main enzyme studied here is highlighted with yellow. (B) A map of genomic DNA displays the Dadi1 (CG32068) locus. The D ADI1d01129 P-element insertion line (black arrow) contains a p-element at 48Â bp upstream of the D ADI1 start codon (red arrow). Three D ADI1 mutant alleles (Dadi17, Dadi19 and Dadi17 4) were produced with alterations in the Dadi1 coding region. (C) Immunoblotting of D ADI1 in several developmental stages. Embryos were collected after egg deposition. (D) The protein levels of D ADI1 were reduced in Dadi174 heterozygote strain (Dadi174/TM3Ser). Western blot analysis of D ADI1 protein levels of Dadi17/ Dadi19 trans-heterozygous alleles and Dadi174 homozygous mutant indicated that they were protein null alleles.