Figure 1

Binding strength of MT-AV to PC-3 cell surface PS. PC-3 cells were incubated with increasing concentrations of biotin labeled MT-AV with total binding (●) measured in the presence of 2 mM Ca²⁺ and non-specific binding (■) measured in the absence of Ca²⁺ with 5 mM EDTA to chelate any residual Ca²⁺. Specific binding (▲) was obtained by subtracting non-specific from total binding. Data presented as mean ± SE (n = 3).