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Table 1 DNA damaging activity of the dialysed conditioned media

From: PID15, a novel 6 kDa secreted peptide, mediates Naja naja venom phospholipase A2 induced apoptosis in isolated human peripheral lymphocytes

Conditioned media (CM)

% DNA fragmentation

Cmlp – 0 min

 

12 kDa dialysate

10 ± 1.24

8 kDa dialysate

9 ± 1.01

2 kDa dialysate

11 ± 1.89

Cmlp – 5 min

 

12 kDa dialysate

8 ± 0.89

8 kDa dialysate

9 ± 0.92

2 kDa dialysate

11 ± 1.97

Cmlp – 10 min

 

12 kDa dialysate

8 ± 0.78

8 kDa dialysate

9 ± 0.85

2 kDa dialysate

27 ± 2.75

Cmlp – 15 min

 

12 kDa dialysate

9 ± 1.11

8 kDa dialysate

18 ± 1.96

2 kDa dialysate

44 ± 3.26@

Cmlp – 20 min

 

12 kDa dialysate

10 ± 1.54

8 kDa dialysate

8 ± 0.76

2 kDa dialysate

15 ± 1.41

  1. The conditioned media obtained at 0 min, 5 min, 10 min, 15 min, 20 min, 25 min and 30 min time periods, referred as cmlp-0, cmlp-5, cmlp-10, cmlp-15 and cmlp-20 respectively, dialysed using membranes of cutoff 2Kd, 8Kd and 12Kd. Dialysate added to fresh normal lymphocytes (1×106) cells, tested for DNA damage. cmlp (conditioned media obtained from lymphocytes treated with NV-PLA2), kDa (kilo Dalton), SD (Standard Deviation).
  2. @p < 0.05 compared to corresponding control conditioned media (cmlp-c-15) as mentioned in Methods. The values expressed are mean of five experiments and are expressed as Mean ± SD.
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Journal of Biomedical Science

ISSN: 1423-0127