|
Conditioned media (CM)
|
% DNA fragmentation
|
|---|
|
Cmlp – 0 min
| |
|
12 kDa dialysate
|
10 ± 1.24
|
|
8 kDa dialysate
|
9 ± 1.01
|
|
2 kDa dialysate
|
11 ± 1.89
|
|
Cmlp – 5 min
| |
|
12 kDa dialysate
|
8 ± 0.89
|
|
8 kDa dialysate
|
9 ± 0.92
|
|
2 kDa dialysate
|
11 ± 1.97
|
|
Cmlp – 10 min
| |
|
12 kDa dialysate
|
8 ± 0.78
|
|
8 kDa dialysate
|
9 ± 0.85
|
|
2 kDa dialysate
|
27 ± 2.75
|
|
Cmlp – 15 min
| |
|
12 kDa dialysate
|
9 ± 1.11
|
|
8 kDa dialysate
|
18 ± 1.96
|
|
2 kDa dialysate
|
44 ± 3.26@
|
|
Cmlp – 20 min
| |
|
12 kDa dialysate
|
10 ± 1.54
|
|
8 kDa dialysate
|
8 ± 0.76
|
|
2 kDa dialysate
|
15 ± 1.41
|
- The conditioned media obtained at 0 min, 5 min, 10 min, 15 min, 20 min, 25 min and 30 min time periods, referred as cmlp-0, cmlp-5, cmlp-10, cmlp-15 and cmlp-20 respectively, dialysed using membranes of cutoff 2Kd, 8Kd and 12Kd. Dialysate added to fresh normal lymphocytes (1×106) cells, tested for DNA damage. cmlp (conditioned media obtained from lymphocytes treated with NV-PLA2), kDa (kilo Dalton), SD (Standard Deviation).
- @p < 0.05 compared to corresponding control conditioned media (cmlp-c-15) as mentioned in Methods. The values expressed are mean of five experiments and are expressed as Mean ± SD.
