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Table 1 DNA damaging activity of the dialysed conditioned media

From: PID15, a novel 6 kDa secreted peptide, mediates Naja naja venom phospholipase A2 induced apoptosis in isolated human peripheral lymphocytes

Conditioned media (CM) % DNA fragmentation
Cmlp – 0 min  
12 kDa dialysate 10 ± 1.24
8 kDa dialysate 9 ± 1.01
2 kDa dialysate 11 ± 1.89
Cmlp – 5 min  
12 kDa dialysate 8 ± 0.89
8 kDa dialysate 9 ± 0.92
2 kDa dialysate 11 ± 1.97
Cmlp – 10 min  
12 kDa dialysate 8 ± 0.78
8 kDa dialysate 9 ± 0.85
2 kDa dialysate 27 ± 2.75
Cmlp – 15 min  
12 kDa dialysate 9 ± 1.11
8 kDa dialysate 18 ± 1.96
2 kDa dialysate 44 ± 3.26@
Cmlp – 20 min  
12 kDa dialysate 10 ± 1.54
8 kDa dialysate 8 ± 0.76
2 kDa dialysate 15 ± 1.41
  1. The conditioned media obtained at 0 min, 5 min, 10 min, 15 min, 20 min, 25 min and 30 min time periods, referred as cmlp-0, cmlp-5, cmlp-10, cmlp-15 and cmlp-20 respectively, dialysed using membranes of cutoff 2Kd, 8Kd and 12Kd. Dialysate added to fresh normal lymphocytes (1×106) cells, tested for DNA damage. cmlp (conditioned media obtained from lymphocytes treated with NV-PLA2), kDa (kilo Dalton), SD (Standard Deviation).
  2. @p < 0.05 compared to corresponding control conditioned media (cmlp-c-15) as mentioned in Methods. The values expressed are mean of five experiments and are expressed as Mean ± SD.