Figure 2

DEPDC1B effects on the induction of cell migration in Rat6 cells and tumor cell invasion was Rac1-dependent. (A) Wound-healing assay. Rat6 cells and Rat6 cells ectopically expressing DEPDC1B were seeded onto plates. After reaching confluency, the cell layer was wounded with a 200-μL pipette tip and incubated for 24 h. (B) Growth curves of Rat6 cells with and without expression of DEPDC1B. Stable Rat6 cell lines were seeded at an original density of 5000 cells per 6 well dishes, and cell numbers were counted at the indicated time. (C) DEPDC1B enhanced cell migration in Rat6 cells. Migration was measured by quantifying the number of cells that had migrated to the lower chamber of a Boyden chamber after 16 h. (D) DEPDC1B enhanced cell invasion in Hep3B/T2 cells. Invasion was measured by quantifying the number of cells that had migrated to the lower chamber of a Boyden chamber after 16 h. (E) Control Rat6 and Rat6 DEPDC1B-expressing cells stably transfected with Rac1, Rac1N17, or Rac1V12 were grown in 10% fetal bovine serum; Rac1 expression levels were determined. (F) Wound-healing assay. The cell lines were seeded on plates, as previously described. After reaching confluency, the cell layer was wounded with a 200-μL pipette tip and incubated for 17 h.