Figure 2From: Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systemsSplenocyte proliferation levels after in vitro stimulation with HPV-16 E7 antigen. The mice were injected intramuscularly thrice at 7-day intervals with different Nano-chitosan (NCS) regimes. One week after final immunization, spleens of individual mice (five per group) were removed and lymphocyte proliferation was evaluated with MTT method. Formazan crystals were dissolved in dimethyl sulfoxide and optical densities were read at 540 nm. Values are the mean ± standard error of the mean for the experiments. *** Indicates statistically significant difference between the NCS-DNA E7 group as determined by one-way ANOVA (P < 0.001) with other groups.Back to article page