Figure 3
From: Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems
Analysis of the cytotoxic activity of CD8 induced by NCS-DNA E7 expressing the HPV-16 E7 antigen. CTL activity of the lymphocytes from immunized mice (five mice per group) was measured at 100:1 E/T ratio by LDH release assay kit (Takara) as described in Methods section. Specific lysis of target cells are shown with nonspecific background lysis subtracted. *** Indicates statistically significant difference between NCS-DNA E7 group as determined by one-way ANOVA (P < 0.001) with other groups. ## shows the statistical significant differences between DNA-E7, NCS-pcDNA3.1 and NCS treatments than the group treated with PBS (P < 0.01).