Figure 6

NKRF overexpression can inhibit H. TB induced IP-10 and IL-8 release in THP-1 cells. THP-1 cells were transfected with NKRF plasmid (p-CMV-NKRF) or control vector (p-CMV-vector) for 24 hours, and then incubated with H. TB (5 μg/ml) for 1 hr. ChIP assays were performed by using p65 or RNA pol II antibodies. The IP-DNA was quantified by q-PCR with primer pairs specific to IP-10 and IL-8 promoter sites. Values were normalized by input DNA. The ChIP assays showed a significantly decreased in p65 (A) and RNA pol II (B) binding to IP-10 (left panel) and IL-8 (right panel) promoter sites in NKRF overexpress THP-1 cells treated with or without H. TB stimulation. Data are means ± SE. *p < 0.01, compared with the group without H. TB treatment; #p < 0.05 compared with vector control group.