Figure 2

Pin1 upregulation was identified in ESCC specimens and correlated with poor prognosis of patients. (A) Pin1 protein of 56 clinical ESCC tumor and corresponding non-tumor tissues was examined (top panel). The bar chart showed the patient number according to the relative Pin1 expression in tumor part which was normalized to the corresponding non-tumor part (bottom panel). (B) Pin1 mRNA level of 42 clinical ESCC tumor and corresponding non-tumor tissues was examined (top panel). The bar chart showed the patient number according to the relative Pin1 mRNA in tumor part which was normalized to non-tumor part (bottom panel). (C) Pin1 expression of 89 ESCC tumors was examined by IHC. Expression was scored according to the percentage of positively stained cells and intensity of staining (top panel). The bar chart showed the patient number according to Pin1 expression by IHC scoring (bottom panel). (D) Overall survival of patients was calculated by Kaplan-Meier method and compared with log-rank test. Patients with high Pin1 expression has lower survival rate (p < 0.001). (E) The overall survival of 55 patients with stage I and II disease were stratified by Pin1 expression. Patients with high Pin1 expression had lower survival rate (p < 0.001).