The formation of autophagosome and amphisome accompanied with EV71 structural protein VP1 and nonstructural protein 2C was detected in EV71 infected SK-N-SH cells. SK-N-SH cells were infected with EV71 (MOI = 10) for 9 hr. (A) The cells were treated with anti-LC3 rabbit polyclonal antibody and either anti-EV71 2C rat polyclonal antibody or anti-EV71 VP1 mouse monoclonal antibody, then incubated overnight at 4°C and investigated under a confocal microscope. Green: LC3; Red: EV71 2C and VP1; Yellow: colocalization of LC3 and EV71 2C or VP1. (B) SK-N-SH cells were treated with MPR rabbit polyclonal antibody and either EV71 2C rat polyclonal antibody or VP1 mouse monoclonal antibody. Green: MPR; Red: EV71 2C and VP1; Yellow: colocalization of MPR and EV71 2C or VP1. (C) The cells with or without EV71 infection were then treated with LC3 mouse monoclonal antibody and LAMP1 mouse monoclonal antibody or mannose-6-phosphate receptor (MPR) rabbit polyclonal antibody. Green: LC3; Red: LAMP1 and MPR; Yellow: colocalization of LC3 and LAMP1 or MPR. Arrow indicates colocalization.