Suppression of EV71 MP4-induced autophagy in mice reduced EV71-related pathogenesis and viral replication. Seven-day-old ICR suckling mice (n = 3, each group) were inoculated intracranially with 3-MA (0.3 mg/mouse) 2 hr before inoculation with EV71 MP4 (5 × 105 pfu/mouse). (A) The body weight and disease symptoms were monitored daily after inoculation for 6 and 7 days, respectively. (B) The mice received the same treatment as in (A), and the brain tissues of the mice were weighed and immersed in DMEM medium containing 2% FBS (with equal volume) at 12 hr p.i., and viral titer was determined by plaque assay. The viral titer was calculated based on the weight of the mice brain. Results are the mean ± stand deviation of each group. PBS treatment was used as the control. (C) Quantitative presentation showed the viral titer detected in infected mice brains with or without 3-MA. Values are the means ± standard deviation of results of three independent experiments. Student’s t test was used *P < 0.05 (compared to the value of EV71 + PBS).