miRNA expression profiles and validation of microarray data by qRT-PCR analysis. A: Hierarchical cluster analysis of differentially expressed miRNAs in placenta from patients with PE. miRNAs in decidua from women with PE (P1, P2, P3, P4 and P5) and healthy pregnant women (N1, N2, N3, N4 and N5). Each row represents a miRNA and each column represents a sample pair of decidua patients with PE and healthy women with normal pregnancies. The color legend indicates high expression (yellow), low expression (blue) or no change in expression (Black). The grey segments represent the amount of miRNA expression is very low or no hybridization signal is detected. The miRNA data were clustered based on their similarities in expression among these cohorts; B: Differentially expressed miRNAs in patients with PE (n = 5) and healthy pregnant women (n = 5). Average log2 expression ratio of all differentially expressed miRNAs (p < 0.01). Blue dots represent the miRNAs with significant (p < 0.05) differential expression of ≥ 2.0-fold changes. Black dots represent the miRNAs with differential expression of >1.0-fold and <2.0-fold changes (p > 0.05); C: qPCR was used to analyze the expression of miR-136, miR-495, miR-16, miR-29b, miR-140-5p, miR-30a, miR-100, miR-494, miR-221, and miR-1207-5p in dMSCs from patients with PE. D: qPCR was used to analyze the expression of miR-136, miR-495, miR-16, miR-29b, and miR-494 in dMSCs from patients with more severe PE (proteinuria, >2108 mg/24 h, systolic blood pressure, >160.4 mmHg; diastolic blood pressure, >110.4 mmHg) and less severe PE (proteinuria, <2108 mg/24 h, systolic blood pressure, <160.4 mmHg; diastolic blood pressure, <110.4 mmHg). Data indicate relative expression following normalization. Values are means ± SE (*p < 0.05; ***p < 0.01). dMSC, decidua-derived mesenchymal stem cell; miRNA, microRNA; PE, pre-eclampsia; qPCR, quantitative polymerase chain reaction.