Figure 5

Validation of miRNA target. A: The luciferase activity of dMSCs was measured after co-transfection with the indicated VEGFA 3′ UTR constructs and miR-16 or its inhibitor for 24 h. B: The luciferase activity of dMSCs was measured after co-transfection with the indicated PPP2R2A 3′ UTR constructs and miR-136 or its inhibitor for 24 h. The results are shown as mean ± SE from three representative independent experiments. *p < 0.05, **P < 0.01 compared with miR-negative. dMSC, decidua-derived mesenchymal stem cell; miRNA, microRNA; PPP2R2A, serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B α isoform; SE, standard error, UTR, untranslated region; VEGFR, vascular endothelial growth factor.