Acetylation of AIRE influences the correct nuclear localization. COS-1 cells were transfected with phMGFP–AIRE wild type (panel A), phMGFP–AIRE Lys102Arg-Lys111Arg-Lys131Arg-Lys133Arg (panel B), phMGFP–AIRE-Lys102Gln-Lys111Gln-Lys131Gln-Lys133Gln (panel C). In contrast to the nuclear localization of wild type AIRE (panel A), the quadruple Arginine mutant was diffused throughout the cytoplasm (panel B). The wild type localization was restored in the quadruple Glutamine mutants (panel C). The nuclei were stained blue with DAPI.