Figure 2From: AIRE acetylation and deacetylation: effect on protein stability and transactivation activityAcetylation of AIRE influences the correct nuclear localization. COS-1 cells were transfected with phMGFP–AIRE wild type (panel A), phMGFP–AIRE Lys102Arg-Lys111Arg-Lys131Arg-Lys133Arg (panel B), phMGFP–AIRE-Lys102Gln-Lys111Gln-Lys131Gln-Lys133Gln (panel C). In contrast to the nuclear localization of wild type AIRE (panel A), the quadruple Arginine mutant was diffused throughout the cytoplasm (panel B). The wild type localization was restored in the quadruple Glutamine mutants (panel C). The nuclei were stained blue with DAPI.Back to article page