Co-precipitation of endogenous SIN3A with HA-AIRE. COS-1 cells, containing endogenous SIN3A, were transfected with plasmid pEF5HA-AIRE and immunoprecipitated using SIN3A antibody. (line 3 and 4). In line 1 and 2 the empty pEF5HA vector and its total lysate are shown. Upper panel: W.B anti SIN3A. Lower panel: W.B anti AIRE HA. W.B.: Western blotting. I.P.: Immunoprecipitated. T.L.: Total Lysate.