Table 1 Primers used in the present study
From: AIRE acetylation and deacetylation: effect on protein stability and transactivation activity
Construct | Oligonucleotidesequence (5'-3') |
|---|---|
AIRE Lys102Gln | Fprimer: CTGGACAGCTTCCCCCAAGATGTGGACCTCAGCCA |
Rprimer: TGGCTGAGGTCCACATCTTGGGGGAAGCTGTCCAG | |
AIRE Lys102Arg | Fprimer: CTGGACAGCTTCCCCAGAGATGTGGACCTCAGCCA |
Rprimer: TGGCTGAGGTCCACATCTCTGGGGAAGCTGTCCAG | |
AIRE Lys111Gln | F-primer: CTCAGCCAGCCCCGGCAGGGGAGGAAGCCCCCG |
R-primer: CGGGGGCTTCCTCCCCTGCCGGGGCTGGCTGAG | |
AIRE Lys111Arg | F-primer: CTCAGCCAGCCCCGGAGGGGGAGGAAGCCCCCG |
R-primer: CGGGGGCTTCCTCCCCCTCCGGGGCTGGCTGAG | |
AIRE Lys131-133Gln | F-primer: CTCCCCACCCAGAGGCAGGCCTCAGAAGAGGCT |
R-primer: AGCCTCTTCTGAGGCCTGCCTCTGGGTGGGGAG | |
AIRE Lys131-133Arg | F-primer: CTCCCCACCAGGAGGAGGGCCTCAGAAGAGGCT |
R-primer: AGCCTCTTCTGAGGCCCTCCTCCTGGTGGGGAG |