Recombinant Lactobacillus plantarum expressing and secreting heterologous oxalate decarboxylase prevents renal calcium oxalate stone deposition in experimental rats

Sasikumar, Ponnusamy; Gomathi, Sivasamy; Anbazhagan, Kolandaswamy; Abhishek, Albert; Paul, Eldho; Vasudevan, Varadaraj; Sasikumar, Sundaresan; Selvam, Govindan Sadasivam

doi:10.1186/s12929-014-0086-y

Journal of Biomedical Science

Table 2 Urinary biochemistry profile

From: Recombinant Lactobacillus plantarum expressing and secreting heterologous oxalate decarboxylase prevents renal calcium oxalate stone deposition in experimental rats

Days^α	Group I (n = 6)	Group II	Group III	Group IV	Group V
Bodyweight (g)
0	140.40 ± 1.24	130.70 ± 3.18	131.01 ± 3.42	133.57 ± 2.66	133.38 ± 2.27
7	173.49 ± 2.34	134.37 ± 2.01^a*	145.59 ± 2.82^a*	142.27 ± 3.70^a*	140.42 ± 2.10^a*
14	218.31 ± 3.32	151.16 ± 2.64	160.88 ± 2.69	164.90 ± 3.38	150.82 ± 3.01
21	240.39 ± 2.75	171.17 ± 3.04	180.17 ± 3.25	190.76 ± 3.39	171.42 ± 3.35
28	261.11 ± 2.87	195.13 ± 3.70^a*	191.80 ± 2.06^a*	220.14 ± 2.68^{a* bc}	201.73 ± 2.51^{a* bc}
Urine pH
0	7.07 ± 0.11	6.83 ± 0.26	6.84 ± 0.19	6.74 ± 0.25	6.54 ± 0.15
7	7.13 ± 0.16	7.08 ± 0.18	6.94 ± 0.20	7.11 ± 0.18	7.29 ± 0.12
14	7.10 ± 0.22	6.94 ± 0.30	6.94 ± 0.24	6.97 ± 0.20	7.02 ± 0.15
21	7.21 ± 0.15	6.44 ± 0.19^a*	6.55 ± 0.12^a*	6.86 ± 0.21^bc	6.94 ± 0.16
28	7.25 ± 0.11	6.09 ± 0.07^a*	6.16 ± 0.08^a*	6.90 ± 0.17^{a* bc}	6.79 ± 0.13^{a* bc}
Uric acid (mg/24 h)
0	0.05 ± 0.01	0.09 ± 0.01	0.06 ± 0.01	0.05 ± 0.01	0.05 ± 0.01
7	0.12 ± 0.01	0.14 ± 0.01	0.16 ± 0.01	0.07 ± 0.01	0.12 ± 0.04
14	0.11 ± 0.02	0.18 ± 0.01^a*	0.19 ± 0.01^a*	0.09 ± 0.01^bc	0.14 ± 0.01^bc
21	0.15 ± 0.02	0.21 ± 0.02^a*	0.25 ± 0.03^a*	0.11 ± 0.03^bc	0.15 ± 0.01^bc
28	0.17 ± 0.02	0.46 ± 0.02^a*	0.39 ± 0.03^a*	0.12 ± 0.01^bc	0.18 ± 0.02^bc
Creatinine (mg/24 h)
0	1.33 ± 0.08	1.39 ± 0.18	1.23 ± 0.15	1.11 ± 0.11	1.16 ± 0.08
7	1.04 ± 0.06	1.50 ± 0.16^a*	1.69 ± 0.26^a*	1.41 ± 0.11^a*	1.73 ± 0.10^a*
14	1.64 ± 0.24	1.73 ± 0.13^a*	2.82 ± 0.29^a*	1.61 ± 0.18^c*	1.95 ± 0.18^c*
21	1.51 ± 0.31	2.71 ± 0.19^a*	3.19 ± 0.22^a*	2.07 ± 0.09^abc*	2.30 ± 0.16^abc*
28	1.77 ± 0.23	3.69 ± 0.30^a*	3.52 ± 0.19^a*	2.52 ± 0.14^abc*	3.07 ± 0.61^ac

^α Data are expressed as mean ± SD. Comparisons are made against Group I (Control)^a , Group II (lithiatic control)^b and Group III (Non-recombinant strain)^c.
^a*^b* and ^c* indicates the mean value is significant at p < 0.05 against group I, II and III correspondingly. n = 6 rats each group.

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ISSN: 1423-0127

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