Figure 3
From: X-Linked thrombocytopenia causing mutations in WASP (L46P and A47D) impair T cell chemotaxis
Expression of WASPL46Por WASPA47Ddoes not rescue the chemotaxis defect of JurkatWASP-KDT cells. A) Vector plots of migration paths of 20 randomly chosen cells from JurkatWASP-KD T cells expressing RFP, WASPR-RFP, WASPRL46P-RFP, WASPRA47D-RFP exposed to a gradient of SDF-1α in the Dunn chamber. Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to JurkatWASP-KD T cells expressing WASPR-RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01.