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Figure 6 | Journal of Biomedical Science

Figure 6

From: X-Linked thrombocytopenia causing mutations in WASP (L46P and A47D) impair T cell chemotaxis

Figure 6

WASPL46Pand WASPA47Dmutants have reduced tyrosine phosphorylation after SDF-1α stimulation. A) HEK293T cells expressing WASPR or mutants (L46P, A47D) (tagged with His tag) together with or without activated form of Cdc42 (Cdc42G12V). Thirty-six hours after transfection, a His-tag pull down assay was performed and analyzed by western blot for WASP (anti-His), Cdc42 and 4G10 (anti-phosphotyrosine antibody). B) Jurkat T cells expressing WASPR or its mutants (L46P, A47D) (tagged with His tag) were stimulated with SDF-1α for 5 min and lysed. His tag pull down assay was performed and analyzed by western blot for phosphorylation of WASP or its mutants (L46P, A47D) using 4G10 antibody.

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