Figure 6From: X-Linked thrombocytopenia causing mutations in WASP (L46P and A47D) impair T cell chemotaxisWASPL46Pand WASPA47Dmutants have reduced tyrosine phosphorylation after SDF-1α stimulation. A) HEK293T cells expressing WASPR or mutants (L46P, A47D) (tagged with His tag) together with or without activated form of Cdc42 (Cdc42G12V). Thirty-six hours after transfection, a His-tag pull down assay was performed and analyzed by western blot for WASP (anti-His), Cdc42 and 4G10 (anti-phosphotyrosine antibody). B) Jurkat T cells expressing WASPR or its mutants (L46P, A47D) (tagged with His tag) were stimulated with SDF-1α for 5 min and lysed. His tag pull down assay was performed and analyzed by western blot for phosphorylation of WASP or its mutants (L46P, A47D) using 4G10 antibody.Back to article page