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Table 1 Data collection of significant fold-changes for detected proteins from Micro-Western Array analysis

From: Arecoline induces TNF-alpha production and Zonula Occludens-1 redistribution in mouse Sertoli TM4 cells

 

Antibodies

Arecoline treated time

10 minutes

60 minutes

Increased

Phospho-p44/42 MAPK (Thr202/Tyr204)

1.98

 
 

Phospho-p38 MAPK (Thr180/Tyr182)

1.51

 
 

Phospho-IkappaB-alpha (Ser36)

 

1.33

 

Phospho-Jun(−c) (Ser63)

 

1.6

 

Phospho-mTOR (Thr2446)

1.16

2.3

Decreased

Phospho-PP2A (Tyr307)

0.89

0.68

 

Phospho-STAT1 (Ser727)

 

0.6

 

Phospho-Src (Tyr418)

0.85

0.79

  1. Note: Relative protein abundance was normalized to the average of GAPDH and actin. A fold change of 1.3 or 0.7 in expression between 0 and 10 minutes or 0 and 60 minutes groups was used as a cutoff.
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Journal of Biomedical Science

ISSN: 1423-0127