| |
Antibodies
|
Arecoline treated time
|
|---|
|
10 minutes
|
60 minutes
|
|---|
|
Increased
|
Phospho-p44/42 MAPK (Thr202/Tyr204)
|
1.98
| |
| |
Phospho-p38 MAPK (Thr180/Tyr182)
|
1.51
| |
| |
Phospho-IkappaB-alpha (Ser36)
| |
1.33
|
| |
Phospho-Jun(−c) (Ser63)
| |
1.6
|
| |
Phospho-mTOR (Thr2446)
|
1.16
|
2.3
|
|
Decreased
|
Phospho-PP2A (Tyr307)
|
0.89
|
0.68
|
| |
Phospho-STAT1 (Ser727)
| |
0.6
|
| |
Phospho-Src (Tyr418)
|
0.85
|
0.79
|
- Note: Relative protein abundance was normalized to the average of GAPDH and actin. A fold change of 1.3 or 0.7 in expression between 0 and 10 minutes or 0 and 60 minutes groups was used as a cutoff.
