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Table 2 Validation of microarray data of selected miRNA-5p and -3p pairs by real-time qRT-PCR

From: MicroRNA-5p and -3p co-expression and cross-targeting in colon cancer cells

miRNA-5p

Log2(fold change)

miRNA-3p

Log2(fold change)

(Mean ± SD)

(Mean ± SD)

 

WGBS

qRT-PCR

 

WGBS

qRT-PCR

miR-20a-5p

7.19 ± 0.78**

7.74 ± 1.17**

miR-20a-3p

1.58 ± 0.98

4.23 ± 1.01**

miR-378a-5p

− 2.60 ± 0.01**

− 1.13 ± 0.87

miR-378a-3p

− 1.04 ± 0.27

− 1.06 ± 1.01

let-7d-5p

2.29 ± 1.01

4.08 ± 0.87**

let-7d-3p

− 2.78 ± 0.79**

− 2.96 ± 0.33**

  1. The mean ± standard deviation (SD) real-time quantitative RT-PCR (qRT-PCR) data were obtained in three independent experiments on all four colon cancer cell lines as described in the text. WGBS, data from the WaferGen Biosystems microarray analysis. **p < 0.01.
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Journal of Biomedical Science

ISSN: 1423-0127