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Table 2 Validation of microarray data of selected miRNA-5p and -3p pairs by real-time qRT-PCR

From: MicroRNA-5p and -3p co-expression and cross-targeting in colon cancer cells

miRNA-5p Log2(fold change) miRNA-3p Log2(fold change)
(Mean ± SD) (Mean ± SD)
  WGBS qRT-PCR   WGBS qRT-PCR
miR-20a-5p 7.19 ± 0.78** 7.74 ± 1.17** miR-20a-3p 1.58 ± 0.98 4.23 ± 1.01**
miR-378a-5p 2.60 ± 0.01** 1.13 ± 0.87 miR-378a-3p 1.04 ± 0.27 1.06 ± 1.01
let-7d-5p 2.29 ± 1.01 4.08 ± 0.87** let-7d-3p 2.78 ± 0.79** 2.96 ± 0.33**
  1. The mean ± standard deviation (SD) real-time quantitative RT-PCR (qRT-PCR) data were obtained in three independent experiments on all four colon cancer cell lines as described in the text. WGBS, data from the WaferGen Biosystems microarray analysis. **p < 0.01.