Pretreatment with EPA or DHA prevents the doxorubicin-induced change in the mitochondrial membrane potential (Δψ) (A) and cell viability (B). H9C2 cells were treated as in Figures 4 and 5, then the Δψ was measured using JC-1 dye and flow cytometry. The results are expressed as the mean ± S.D. for three separate experiments. **, ***: p <0.01 and p <0005 compared to the untreated; #, ##: p <0.05 and p <0.01 compared to doxorubicin treatment.