Figure 6

Inhibition of corneal neovascularization by miRNAs in a corneal injury animal model. Alkali burn-induced corneas of Sprague–Dawley rats were treated once with each miRNA mimic by subconjunctival injection on the day of injury. (A-C) Photographic images of corneas treated with each miRNA mimic. Immunofluorescent stain of corneal sections to detect vessel formation (D-F) or lymphangiogenesis (G-I). Red stains F-actin (D-F) or LYVE-1 (G-I), which is a marker of vessels and lymphatic vessels, and blue shows hoechst staining (D-I). Photographs of corneas were taken two weeks after the treatment. (J) Quantification of new blood vessel formation, F-actin-stained area (K), and LYVE-1-stained area (L). Relative rate was quantified using ImageJ software and normalized to the value obtained from the scrambled control-injected rats. Error bars indicate SDs. †P < 0.01.