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Figure 2 | Journal of Biomedical Science

Figure 2

From: Effects of tobacco smoking during pregnancy on oxidative stress in the umbilical cord and mononuclear blood cells of neonates

Figure 2

Flow cytometry analysis of apoptosis. Dot plots show apoptosis ratios of umbilical cord mononuclear blood cells from (A) non-smokers (n = 13, left panel) and smokers (n = 13, right panel) using propidium iodide (PI) and FITC-annexin V. The Q1 quadrant represents unviable cells (PI positive and annexin negative). The Q2 quadrant represents cell that are in late apoptosis or necrosis (both annexin and PI positive). The Q3 quadrant represents viable cells (both annexin and PI negative). The Q4 quadrant represents cells in early apoptosis/cell apoptosis (annexin positive and PI negative). Note the remarkable increase in apoptotic cells number (Q2 + Q4) in the smokers group (n = 13). (B) Bar graph shows average percentage of apoptotic cells (Q2 + Q4). The values are presented as means ± SEM. *p <0.05 vs. non-smokers.

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