Expression of EpCAM & binding of the complexes on cells. A. Histogram overlay plot showing the expression levels of EpCAM protein in MCF-7 cells were evaluated using antibody based method and flow cytometry. B. Histogram overlay plot of MCF-7 cells bound to EpApt/EpDT3 or ScrApt/ScrDT3. C. Histogram overlay plot of MCF-7 cells bound to aptamer alone or PEI-Apt-siRNA complexes. D. Histogram overlay of WERI-Rb1 cells. Aptamer alone or complexes were added to cells and incubated for 2 h, washed & checked by flow cytometry. E. Flow cytometry analysis and histogram overlay plot showing the cells blocked with EpCAM antibody before incubating with EpDT3-FI or with EpDT3-FI alone and unstained cells. F. Flow cytometry analysis and histogram overlay plot of cells blocked with antibody followed by incubation with PEI-EpApt-SiEp.