Flow cytometry analysis to determine the ability of bortezomib and/or SAHA pretreated TC-1 cells to induce IFN-γ
secretion in E7-specific cytotoxic T cells. TC-1 cells were plated in 6-well plates (2 × 105 /well) and treated with bortezomib (3.5 nM) and/or SAHA (12.5 μM) for 24 h. The cells were then harvested and 1 × 105 tumor cells/well were co-incubated with E7-specific CD8+ T cells in 96-well for 4 h (E:T ratio of 1:1) in the presence of GolgiPlug. The cells were then harvested and stained with PE-conjugated CD8 and FITC-conjugated IFN-γ. Cells were acquired with FACSCalibur and analyzed with FlowJo. A. Representative flow cytometry data demonstrating the percentage of IFN-γ positive cells among E7-specific CTLs induced by TC-1 cells treated with bortezomib and/or SAHA. B. Bar graph depicting the percentage of IFN-γ + E7-specific CTLs among all E7-specific CTLs induced by TC-1 cells treated with bortezomib and/or SAHA.