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Figure 5 | Journal of Biomedical Science

Figure 5

From: Combination of proteasome and HDAC inhibitor enhances HPV16 E7-specific CD8+ T cell immune response and antitumor effects in a preclinical cervical cancer model

Figure 5

In vitro cytotoxic T cell assay. Luciferase-expressing TC-1 tumor cells were added to 96-well plates (1 × 104 per well) and incubated for 18 h at 37°C. The cells were then treated with bortezomib (3.5 nM) and/or SAHA (12.5 μM) for 24 h. After washing with PBS, HPV16 E7-specific CTLs were added to the cells and incubated at 37°C for 4 hours. The expression of luciferase was measured using the IVIS luminescence imaging system series 2000. Bioluminescence signals were acquired for 30 s. A. Representative luminescence images of 96-well plates showing tumor cell lysis. B. Line graph depicting the quantification of luminescence intensity in tumor cells treated with drugs and/or E7-specific CTLs in different E:T ratio data shown as mean ± SE.

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