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Figure 1 | Journal of Biomedical Science

Figure 1

From: Aberrant gene expression profiles, during in vitro osteoblast differentiation, of telomerase deficient mouse bone marrow stromal stem cells (mBMSCs)

Figure 1

Telomerase deficient BMSCs exhibited impaired in vitro osteoblast differentiation and proliferation with reduced population doubling time and level. A) Short term proliferation of Terc -/ and WT BMSCs, equal number of cells were plated at day 0 and counted at day 1, 3 and 7. B) Long term proliferation of Terc -/- and WT BMSCs. Population doubling time (PDT); Terc -/- BMSCs PDT of 2.1 days compared to PDT of 1.89 days by WT BMSCs at early passage and later increased to 23 and 3.8 days, respectively. C) In vitro osteoblast differentiation of Terc -/- and WT BMSCs - according to the protocol described in material and methods. Alkaline phosphatase (ALP) (upper row) and Alizarin Red (lower row) staining at day 10 of osteoblast differentiation. Data are represented of means ± SD of three independent experiments. *p ≤ 0.05.

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