Figure 2

Differential gene expression profiles of telomerase deficient BMSCs employing mouse osteogenesis RT ² profiler™ PCR array. A) Pei chart showing differential gene expression profiles associated with each stage of osteogenesis. B) Differentiation gene expression profiles of transcription factors and regulators in Terc ^-/- BMSCs up-regulated (red bars) and down-regulated (green bars) relative to WT controls. C-E) Skeletal development genes, known to be involved in bone mineralization, cartilage condensation and ossification, up-regulated (red bars) and down-regulated (green bars) in Terc ^-/- BMSCs relative to WT controls. Genes that were not amplified or not detectable in the PCR array were marked as ‘n.d’ (not detectable). Osteogenic super array data are represented as fold change relative to WT controls of three independent biological replicates pooled together. Panel (A) C = control, I = induced, scale bar 500 μm.