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Figure 3 | Journal of Biomedical Science

Figure 3

From: Differential regulation of protein expression in response to polyunsaturated fatty acids in the liver of apoE-knockout mice and in HepG2 cells

Figure 3

Effects of IL-1β and IL-6 on CRP gene transcription. HepG2 cells were co-transfected with a CRP promoter reporter construct and a β–galactosidase construct. After transfection for 24 h, the cells were treated with 10 ng/mL of IL-1β/IL-6 (A), with10 ng/mL of IL-1β alone (B) or with 10 ng/mL of IL-6 alone (C) for an additional 12 h. The cell lysates were processed and the luciferase activity was normalized against β-galactosidase activity in order to correct for transfection efficiency. Relative luciferase units are indicated as the relative value of the activity of the untreated group transfected with the pGL3-basic vector (set as 1). Results are presented as the mean ± SEM of at least three independent experiments. *P < 0.05 compared to the IL-6-treated group, **P < 0.01 compared to the vehicle-treated group.

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