Figure 4

Effects of PUFAs on IL-6-induced NF-κB subunit translocation to the nucleus in HepG2 cells. Cells were pretreated with 100 μM PUFAs for 24 h before stimulation with 10 ng/mL of IL-6 for an additional 30 min. The levels of nuclear NF-κB subunits p65 (A) and p50 (B) were detected by western blot analysis. The expression of B23 was used as the internal control for the nuclear protein. Data were quantified by densitometry and the value of the IL-6-treated group was set as 100%. Results are presented as the mean ± SEM of at least three independent experiments, **P < 0.01 compared to the IL-6-treated group.