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Table 1 Primer sequences employed in the analyses of HLPt -mRNA expression and respective amplicon sizes

From: Effects of human blood red cells on the haemolytic capability of clinical isolates of Candida tropicalis

Gene

Orientation

Sequence (5′ → 3′)

Amplicon size

Reference

HLPt*

FW

TAGTGGGGCAAGTAGTGGG

151 pb

This study

RV

GGTGGTGATAGATGTAGCAG

Β-Actin

FW

AACCTCTTCTCAATCATCTGC

129 pb

Vandeputte et al. [25]

RV

GCTTCCAAACCTAAATCGGC

  1. *HLPt primers were designed based on the complete ORF of the putative C. tropicalis haemolysin-like protein gene sequence using Gene Runner 3.05 software.
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Journal of Biomedical Science

ISSN: 1423-0127