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Table 2 Impact of inoculating bacteria without or with 5% porcine mucin in the model of pneumonia in neutropenic mice with diverse strains of penicillin non susceptible Streptococcus pneumoniae (PNSSP)

From: A strain-independent method to induce progressive and lethal pneumococcal pneumonia in neutropenic mice

 

Strain of Penicillin Non Susceptible Streptococcus pneumoniae

Parameter

ATCC 49619

INS-E611

INS-E674

INS-E676*

INS-E678*

INS-E683

INS-E684

Inoculum preparation: Todd Hewitt broth (THB) without or with mucin

THB

Mucin + THB

THB

Mucin + THB

THB

Mucin + THB

THB

Mucin + THB

THB

Mucin + THB

THB

Mucin + THB

THB

Mucin + THB

Inoculum (mean, log 10 CFU/mouse)

7.12

6.34

6.95

6.78

6.46

6.79

6.28

6.20

6.53

6.53

6.71

6.61

7.00

6.25

Time to nadir (h)

14

14

14

14

14

14

**

**

**

**

14

14

14

14

Time to zenith (h)

42

38

36

38

48

38

***

***

***

***

36

38

48

38

Bacterial count at nadir (mean log 10 CFU/mouse ± SD)

5.82 ± 0.09

6.32 ± 0.52

5.43 ± 0.39

5.35 ± 0.53

6.52 ± 0.65

7.34 ± 0.43

4.30 ± 0.36

3.97 ± 0.43

4.93 ± 0.06

6.02 ± 0.13

4.90 ± 0.17

5.70 ± 0.39

7.01 ± 0.72

6.73 ± 0.74

Bacterial count at zenith (mean log 10 CFU/mouse ± SD)

8.02 ± 0.05

6.79 ± 0.44

7.58 ± 0.35

9.26 ± 0.19

8.70 ± 0.51

9.15 ± 0.34

5.09 ± 0.56

7.51 ± 0.18

6.68 ± 0.04

8.67 ± 0.28

7.77 ± 0.53

8.91 ± 0.75

9.59 ± 0.53

9.01 ± 0.52

Growth from nadir to zenith (mean)

2.19

0.47

2.15

3.91

2.18

1.63

---

---

---

---

2.87

3.21

2.58

2.28

Growth from hour 14 to 38 (mean)

1.24

0.47

−0.43

3.91

1.89

1.63

0.79

3.54

1.75

2.65

1.83

3.21

1.94

2.28

Mouse Lethality (%)

0

100

0

100

67

100

0

100

0

100

0

100

100

100

Time to death (mean, h)

All alive

64

All alive

52

56

52

All alive

44

All alive

44

All alive

59

61

51

Bacterial count after death from pneumonia and sepsis (mean ± SD)

All alive

8.21 ± 0.51

All alive

8.37 ± 0.39

9.52 ± 0.01

9.00 ± 0.30

All alive

7.89 ± 0.43

All alive

8.92 ± 0.05

All alive

8.52 ± 0.62

8.22 ± 0.38

8.76 ± 0.04

  1. *All PNSSP strains were used in at least in two separate experiments, except for E676 and E678, which were tested once.
  2. **Time fixed at 14 h.
  3. ***Time fixed at 38 h.