Figure 4

Effect of PLCA and metformin on cell death in NRVM after H/R stress. Progression of cell death was measured in NRVM after H/R stress. (A) Original microscopy photos were reported. Cells indicated green are in early apoptosis (Annexin V-FITC positive and PI negative). Cells indicated orange are in late apoptosis or already dead (annexin V-FITC positive and PI positive). (B) Ratio of early apoptosis cell. (C) Ratio of late apoptosis cell. (n = 5) #P < 0.05 vs. H/R + vehicle.