Colocalization of PKCδ and LCN2 in neutrophils. Neutrophils treated with or without fMLP were stained with specific antibodies against PKCδ (red) and LCN2 (green). Merged images (yellow) indicate colocalization of PKCδ and LCN2. Phalloidin staining (dark blue) revealed the reorganization of F-actin and polarization of neutrophils. An arrow indicates the front edge of the neutrophil that is enriched with F-actin. DAPI (light blue) was used to detect nuclei. Scale bars, 10 μm.