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Figure 1 | Journal of Biomedical Science

Figure 1

From: Generation of CCR5-defective CD34 cells from ZFN-driven stop codon-integrated mesenchymal stem cell clones

Figure 1

Primers map and donor plasmid generation. Illustration of the CCR5 locus and primer locations. Upper panel: ZFN-targeted DNA sequence (underlined) and the interspace. Lower panel: DNA sequences of the d-plasmid around the ZFN site and the insertion-specific primer (IP); a silent T-A mutation adjacent to the left ZFN-targeted site was intentionally selected for confirmation and marking of the vicinity of integration between the ZFN-binding sites.

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Journal of Biomedical Science

ISSN: 1423-0127