Multidrug-resistant phenotype and isolation of a Novel SHV- beta-Lactamase variant in a clinical isolate of Enterobacter cloacae

Table 1 Sequences of the primers used to detect β-lactamases genes

PCR target	Primer name	Primer sequence 5′ → 3′	Annealing T°	Ampliquons size
CTX-M	CTX-M-A	CGCTTTGCGATGTGCAG	54	539
CTX-M	CTX-M-B	ACCGCGATATCGTTGGT	54	539
SHV	SHV-1 F	ATGCGTTATATTCGCCTGTGTAT	54	868
SHV	SHV-1R	TTAGCGTTGCCAGTGCTCGATCAG	54	868
TEM	TEM-A2	GTATCCGCTCATGAGACAAT	54	932
TEM	TEM-ext	GTATATATGAGTAAACTTGGTCTG	54	932
OXA	OXA-1 F	CACAATACATATCAACTTCGC	54	793
OXA	OXA-1R	GTGTGTTTAGAATGGTGATCGC	54	793

ISSN: 1423-0127