SHP deficiency blunts TZD effects on lipid profile of ob/ob mice. (A) Histology of livers from ob/ob and ob/ob;Shp
−/− mice treated with control or troglitazone for 2 weeks. H&E staining was performed for liver sections. ×100 magnification. (B-E) 7–8 week-old male ob/ob and ob/ob;Shp
−/− mice were treated with control (open bars) or troglitazone (filled bars) for 2 weeks. (B) Ratio of liver weight to body weight (n = 10–15 per group). (C) Liver triglyceride (TG) content was determined in liver extracts of ob/ob and ob/ob;Shp
−/− mice (n = 4–5 per group). (D, E) Serum TG and free fatty acid (FFAs) contents under fasting conditions (n = 10–12 and n = 4–5 per group, respectively). Data are mean ± SEM. *P < 0.01, **P < 0.05, for differences within each genotype between control and troglitazone-treated mice. #P < 0.01, ##P < 0.05 for differences between control-treated ob/ob;Shp
−/− and control-treated ob/ob mice.