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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Hydrogen sulfide protects spinal cord and induces autophagy via miR-30c in a rat model of spinal cord ischemia-reperfusion injury

Fig. 4

Targeting site of miR-30c in the Beclin-1 3’UTR. (a) Beclin-1 3’UTR was predicted a binding site for hsa-miR-30c. The highly conserved mature miR-30c sequence and potential binding between the miR-30c seed region to the mouse Beclin-1 3’UTR sequence are shown. 24 h after transfected with pre-miR-30c or anti-30c, SY-SH-5Y cells were lyzed to analyze (b) Beclin-1 3’UTR activity by using luciferase report assay, (c) Beclin-1 mRNA and (d) LC3 mRNA by using Quantitative Real-Time PCR. All values are expressed as the mean ± SD. * P < 0.05 vs. normoxia negative control group; # P < 0.05 vs. ischemic negative control group

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