Correlation between MFI × POI with MOI of Ad-CMV-GFP in A549 cells with flow cytometer adjusted. Transduction at MOI 0.01 to 200 for 18 and 36 h. The sensitivity of the flow cytometer was adjusted to prevent the overshooting of a strong GFP signal. (A-C) GFP signals and cell count of control cells (A) and cells infected for 18 and 36 h (B and C). (D-G) Correlation between MFI × POI and MOI. R2, coefficient of determination.