Fig. 3

Effects of sulforaphane and MG132 on the stability of EGFR in NSCLC cells. (a) Cells were treated with or without 10 μM sulforaphane for the indicated durations in the presence of 40 μg/ml cycloheximide, and the level of tEGFR was determined by Western blotting. β-actin was used as the loading control. The relative levels of tEGFR at different times were quantified by densitometer and were shown below tEGFR. (b) H1975 cells were treated with 10 μM sulforaphane for 24 h in the presence or absence (control) of 10 μM MG132. The levels of tEGFR were assessed by Western blotting. β-actin was used as the loading control. The relative levels of tEGFR were quantified and shown below tEGFR. The data shown are representative of three experiments with similar results