The icv-MPP+-injection-induced lost of dopaminergic neurons in SNpc were marginally attenuated by icv-LSA-injection. Two μl vehicle, MPP+ (12 μg) alone or MPP+ (12 μg) combined with LSA (5 μg) were injected into lateral ventricle of ICR mice. The mice were sacrificed 5 days later. Brain of the mice was subjected to cryosection and immunostained by anti-tyrosine hydrolase antibody (green). Panel (a) is the representative fluorescence images. Panel (b) is the quantification of tyrosine hydrolase immunoreactivity. Results are means ± S.D. from three independent experiments. Significant differences between the mice treated with vehicle and MPP+ were indicated by **, P < 0.01; ***, P < 0.001. Significant differences between the cells treated with 300 μM MPP+ alone and 300 μM MPP+ combined with LSA were indicated by ##, P < 0.01.