Fig. 7

The icv-MPP⁺-injection-induced activation of glial cells in SNpr and CA1 were attenuated by icv-LSA-injection. 2 μl vehicle, MPP⁺ (12 μg) alone or MPP⁺ (12 μg) combined with LSA (5 μg) were injected into lateral ventricle of ICR mice. The mice were sacrificed 5 days later. Brain of the mice was subjected to cryosection and immunostained by anti-GFAP antibody (green) and anti-Iba-1 antibody (red). Panel (a) and (b) are the representative fluorescence images in SNpr/SNpc/PoDG and CA1 area, respectively. The right part of the panels is the amplified images of the dotted square in the left part of the panel. Panel (c) and (d) is the quantification of GFAP and Iba-1 immunoreactivity of panel (a) and (b), respectively. Results are means ± S.D. from three independent experiments. Significant differences between the mice treated with vehicle and MPP⁺ were indicated by *, P < 0.05; **, P < 0.01; ***, P < 0.001. Significant differences between the mice treated with MPP⁺ alone and MPP⁺ combined with LSA were indicated by ##, P < 0.01; ###, P < 0.001