Fig. 5

Inhibition of Aurora kinases A and B by midostaurin. The effect of midostaurin on Aurora kinases A and B was examined comparing with that of VX-680. a MDA-MB-468 cells were treated with midostaurin or VX-680 at indicated concentrations for 3 h, and then subjected to Western blot analysis using the antibodies as in Fig. 4. b In vitro kinase activity of Aurora kinases A and B was measured in the presence of midostaurin or VX-680 at indicated concentrations, and the results were shown as a ratio relative to the control sample in the absence of the inhibitor. Bars are 1 s.d. of duplicate experiments